Partial characterization of a major autolysin from Mycobacterium phlei

Partial Purification of Topoisomerase I from Mycobacterium phlei*

Aim: DNA topoisomerases control several cellular activities by catalyzing the relaxation of superhelices, which are produced during the replication of DNA. Investigation of the inhibitory action of the candidate substances on topoisomerase activity is widely used in drug development. The purpose of this study was to purify topoisomerase I from Mycobacterium phlei, which is closely related to My...

Isolation from Mycobacterium Phlei

Mycobactin is the name suggested for a growth factor, present in acid-fast bacteria, which is essential for the growth of Mycobacterium johnei. A preliminary account ofthe work reported here has already been published (Francis, Madinaveitia, Macturk & Snow, 1949). Myco. johnei is an organism first observed by Johne & Frothingham (1895) in cases of chronic enteritis of cattle. It could not be cu...

Purification and characterization of dihydrofolate reductase from Mycobacterium phlei.

The dihydrofolate reductase from Mycobacterium phlei was purified and characterized; it has an Mr of 15 000 and a pI of 4.8. It is competitively inhibited by both methotrexate and trimethoprim, although the affinity is less than for other bacterial dihydrofolate reductases.

Genetic transfer in Mycobacterium phlei.

Histones and histone-like DNA-binding proteins: correlations between structural differences, properties and functions.

A low-molecular-mass protein from Methylococcus capsulatus (Bath) is responsible for the regulation of formaldehyde dehydrogenase activity in vitro S. Tate and H. Dalton Partial characterization of a major autolysin from Mycobacterium phlei 2. S. Li, T. 1. Beveridge, J. Betts and A. 1. Clarke Oxidation of medium-chain acyl-CoA esters by extracts of Aspergillus niger: enzymology and characteriza...